Abstract—This study employs a NanoBioArray (NBA) chip for single base-pair discrimination between the sequence of the wild type Kras gene and three of its common codon 12 mutants, which are frequently observed in the adenocarcinoma diseases. Here we use gold nanoparticles (AuNPs) in order to perform the single nucleotide polymorphism (SNP) assay without the need of temperature stringency. In the NBA chip, an array of 20-mer oligonucleotide probes was first created on the glass surface, and then a trace amount (2.5 fmol) of target oligonucleotides (20-mer and 60-mer) were introduced through the PDMS microchannels. DNA hybridization between the targets and their complementary probes was achieved at the intersections between microchannels and probe lines. The target oligonucleotides were previously loaded on the AuNP surfaces which established a competition between the comlementary probes and the AuNP surface for binding to the targets. This competition, in turn, enhances the hybridization stringency which disfavor sthe mismatch binding thus facilitates the SNP detection abilities. Using this technique fast and high-throughput multiple discrimination of Kras gene codon 12 was achieved at room-temperature using the NBA chip and the specificity of the method was proved to be as high as the temperature stringency method.

Index Terms—single nucleotide polymorphism (SNP), kras gene, gold nanoparticle, target DNA, microarray, NanoBioArray chip.

Cite: Abootaleb Sedighi and Paul C. H. Li, "Gold Nanoparticle Assists SNP Detection at Room Temperature in the NanoBioArray Chip," International Journal of Materials Science and Engineering, Vol. 1, No. 1, pp. 45-49, June 2013. doi: 10.12720/ijmse.1.1.45-49