Volume 10, No. 3, September 2022
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Preparation of Nanomaterial-Based Artificial Enzymes Using Integrated Graphitic Carbon Nitride Nanosheet-encapsulated Metal-Organic Framework Optimized by Central Composite Design for Enhanced Electrochemiluminescence Biosensing of Gene-Specific Methylati

Seyyed Mehdi Khoshfetrat1, Farzaneh Moradnia1,2, Salman Jalalifar3,4
1.Department of Chemistry, Faculty of Basic Science, Ayatollah Boroujerdi University, Boroujerd, Iran.
2.Department of Chemistry, University of Zanjan, P O Box 45195-313, Zanjan, Iran.
3.School of Engineering, Faculty of Science and Engineering, Macquarie University, Sydney, NSW, Australia.
4.Australian Maritime College, College of Sciences and Engineering, University of Tasmania, Launceston, Tasmania, Australia.
Abstract: Herein, a sensitive electrochemiluminescence immuno-DNA sensor was designed to analyze DNA methylation using sandwiching the target methylated DNA between the magnetic nanoparticles/Anti-5-methylcytosine monoclonal antibody bioconjugate and luminol-loaded within phosphorylated DNA capture probe-immobilized graphitic carbon nitride nanosheets @UiO-66 core@shell nanozyme. The suggested method was successfully utilized to evaluate methylated DNA in human blood plasma, demonstrating the platform's potential for disease diagnostics and biochemistry research. Our method can differentiate methylation levels as low as 0.1%.

Key words: C3N4@UiO-66 core@shell, electrochemiluminescence, nanomaterial-based artificial Enzymes

Cite: Seyyed Mehdi Khoshfetrat, Farzaneh Moradnia, Salman Jalalifar, "Preparation of Nanomaterial-Based Artificial Enzymes Using Integrated Graphitic Carbon Nitride Nanosheet-encapsulated Metal-Organic Framework Optimized by Central Composite Design for Enhanced Electrochemiluminescence Biosensing of Gene-Specific Methylation in Thyroid Cancer Patients' Plasma," International Journal of Materials Science and Engineering, Vol. 10, No. 3, pp. 57-62, September 2022. doi: 10.17706/ijmse.2022.10.3.57-62.

Copyright © 2022 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

 

General Information

ISSN: 2315-4527 (Print)
Abbreviated Title: Int. J. Mater. Sci. Eng.
Editor-in-Chief: Prof. Emeritus Dato' Dr. Muhammad Yahaya
DOI: 10.17706/ijmse
Abstracting/ Indexing: Ulrich's Periodicals Directory, Google Scholar, Crossref
E-mail: ijmse@iap.org